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RNA EMSA Mobility Shift Assay

RNA EMSA Mobility Shift Assay - RNA Techniques Forum

RNA EMSA Mobility Shift Assay - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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  #1  
Old 03-18-2006, 01:05 AM
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Arrow RNA EMSA Mobility Shift Assay



I am trying to do an RNA Shift assay...

are there any non-radioactive methods for EMSA?
I dont want to use p32!

thanks...
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Old 06-19-2006, 08:21 PM
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Default Re: RNA EMSA Mobility Shift Assay

There are many ways to do non-radioactive RNA EMSA.

the best way is using the Pierce Lightshift Chemiluminescent Kit
http://www.piercenet.com/Products/Br...fldID=06010701
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Old 11-11-2006, 02:30 PM
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Default Re: RNA EMSA Mobility Shift Assay

hello everyobody!
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Old 11-11-2006, 10:06 PM
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Default Re: RNA EMSA Mobility Shift Assay

Hey there Mustard.

Yeah the Pierce kit is pretty good. Hope they decrease their prices though as the kit is about $700.

I usually reuse most of the reagents and they still work after 1-4 times reuse.
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Old 05-15-2008, 10:01 PM
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Default Re: RNA EMSA Mobility Shift Assay

does anyone have the experience how to get the biotin labeled DNA and how to measure the concentration of DNA?

do you have any tricks about handling the biotin labeled DNA?

thanks
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assay , emsa , mobility , rna , shift


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