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RNA Extraction Protocols

RNA Extraction Protocols - RNA Techniques Forum

RNA Extraction Protocols - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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  #1  
Old 05-19-2008, 04:41 PM
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Cool RNA Extraction Protocols



Hello,
does anyone have any RNA Extraction Protocols for animal tissues?

thanks
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Old 05-19-2008, 06:33 PM
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Default Re: RNA Extraction Protocols

[Only registered users see links. ]
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Old 07-02-2008, 01:40 PM
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Default Re: RNA Extraction Protocols

If you don't like to mess with phenol/chloroform and like an easier and simple protocol, try [Only registered users see links. ].

1) Homogenize 20mg tissue in 200ul AquaRNA.
2) Spin to pellet debris and transfer super to a new tube.
3) Mix with 1vol of isopropanol and spin to pellet RNA and DNA (keep the super if you want to recover the proteins for Western).
4) Rinse the pellet with 70% EtOH, air dry, suspend pellet in 50ul water.

Do a DNaseI digest if you need to remove the DNA.

Last edited by aquaplasmid; 07-02-2008 at 01:49 PM.
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