RNA Extraction Protocols

dave2006 · #1 (**permalink**) 05-19-2008, 05:41 PM

Hello,
does anyone have any RNA Extraction Protocols for animal tissues?

thanks

danfive · #2 (**permalink**) 05-19-2008, 07:33 PM

[Only registered users see links. ]

aquaplasmid · #3 (**permalink**) 07-02-2008, 02:40 PM

If you don't like to mess with phenol/chloroform and like an easier and simple protocol, try [Only registered users see links. ].

1) Homogenize 20mg tissue in 200ul AquaRNA.
2) Spin to pellet debris and transfer super to a new tube.
3) Mix with 1vol of isopropanol and spin to pellet RNA and DNA (keep the super if you want to recover the proteins for Western).
4) Rinse the pellet with 70% EtOH, air dry, suspend pellet in 50ul water.

Do a DNaseI digest if you need to remove the DNA.

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Plant Protein Extraction the Hard Way	danfive	Protein Science	0	04-24-2008 09:53 PM
Problems with RNA extraction or QPCR for AI	deki	Real-Time PCR and Quantitative PCR Forum	2	12-30-2007 08:58 PM
HUGE Protocols Update	admin	Molecular Biology News and Announcements	1	12-13-2007 09:52 AM
Problems with RNA extraction or QPCR for AI	deki	Basic Lab Protocols and Techniques	3	07-27-2007 03:59 AM
Notice for stem cell protocols	admin	Stem Cell Forum	0	02-03-2006 03:45 AM

Member Login

RNA Extraction Protocols

RNA Extraction Protocols - RNA Techniques Forum

RNA Extraction Protocols - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.