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Minimum Amount of RNA to be Visible on Agarose Gel?

Minimum Amount of RNA to be Visible on Agarose Gel? - RNA Techniques Forum

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Old 04-16-2008, 09:06 AM
Pipette Filler
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Default Minimum Amount of RNA to be Visible on Agarose Gel?



Hello all,

does anyone know what is the lowest concentration of RNA which is needed for RNA to be visible on an agarose gel?
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Old 07-28-2008, 08:32 AM
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Default Re: Minimum Amount of RNA to be Visible on Agarose Gel?

I have used 1ug total RNA to run and seen on gel with EtBr stain
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Old 07-31-2008, 05:26 PM
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Default Re: Minimum Amount of RNA to be Visible on Agarose Gel?

Both molar concentration and length of RNA fragment will influence visibility. If you load the gel with a known mass of RNA instead of by a volume of some molar concentration, you can determine the mass needed for visibility and use that for subsequent experiments. However, a particular volume and concentration of a short sequence of RNA will have less bases for a base-associated dye (e.g. EthidiumBr) to bind with than the same volume and concentration of a long sequence of RNA; therefore keeping volume constant, compared to the longer sequence you will need a higher concentration of the short sequence in order to see the band.
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