Re: Minimum Amount of RNA to be Visible on Agarose Gel?
Both molar concentration and length of RNA fragment will influence visibility. If you load the gel with a known mass of RNA instead of by a volume of some molar concentration, you can determine the mass needed for visibility and use that for subsequent experiments. However, a particular volume and concentration of a short sequence of RNA will have less bases for a base-associated dye (e.g. EthidiumBr) to bind with than the same volume and concentration of a long sequence of RNA; therefore keeping volume constant, compared to the longer sequence you will need a higher concentration of the short sequence in order to see the band.
__________________ Jon D. Moulton, Ph.D.
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