Anyone knows how to precipitate RNA from 100% formamide deionzes solution?

piaojian · #1 (**permalink**) 01-24-2008, 10:53 AM

Help

Who can provide a detailed protocol to do it?

thank you very much!

xenik · #2 (**permalink**) 02-01-2008, 07:46 PM

try to put your samples at 0 C for 1 hour or more(RNA is stable in formamide) than to centrifugate at 16000 g (4 C) for 10-15 min, and remove the supernatant - pellet should be your rna. To visualize pellet you can add some GlycoBlue (Ambion) to your sample before everything (it also important if working with small amounts of rna). I used to do it with 80% formamide - think it would work in your case, otherwise - just add TE buffer to your samples to make it 80% formamide))

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Anyone knows how to precipitate RNA from 100% formamide deionzes solution?

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