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Anyone knows how to precipitate RNA from 100% formamide deionzes solution?

Anyone knows how to precipitate RNA from 100% formamide deionzes solution? - RNA Techniques Forum

Anyone knows how to precipitate RNA from 100% formamide deionzes solution? - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 01-24-2008, 11:53 AM
Pipette Filler
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Default Anyone knows how to precipitate RNA from 100% formamide deionzes solution?



Help

Who can provide a detailed protocol to do it?

thank you very much!
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Old 02-01-2008, 08:46 PM
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Default Re: Anyone knows how to precipitate RNA from 100% formamide deionzes solution?

try to put your samples at 0 C for 1 hour or more(RNA is stable in formamide) than to centrifugate at 16000 g (4 C) for 10-15 min, and remove the supernatant - pellet should be your rna. To visualize pellet you can add some GlycoBlue (Ambion) to your sample before everything (it also important if working with small amounts of rna). I used to do it with 80% formamide - think it would work in your case, otherwise - just add TE buffer to your samples to make it 80% formamide))
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