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RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.

RNA Techniques Forum
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marquage de l'ARN

marquage de l'ARN - RNA Techniques Forum

marquage de l'ARN - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.



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  #1 (permalink)  
Old 12-03-2007, 07:38 PM
Pipette Filler
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Default marquage de l'ARN

Bonjour, je suis étudiante en master 2 biologie moléculaire et cellulaire, spécialité biochimie, et dans le cadre de mon stage j'ai besoin de marquer mon ARNt sur son extremité 3' afin de pouvoir le visualiser au cours de mes manip, pourriez vous me conseiller sur des marqueurs fluorescent en 3' ou un autre moyen de marquage sans toucher a la radioactivité.
je vous remercie d'avance.
LILA
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  #2 (permalink)  
Old 12-03-2007, 09:19 PM
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Default Re: marquage de l'ARN

Fluorophores pour l'incorporation dan ARN: flourescein, hexachlorofluorescein, rhodamine, cyanine

Digoxigenin pour la détection par les anticorps d'anti-Digoxigenin
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