Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > RNA Techniques Forum
Register Search Today's Posts Mark Forums Read

RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Running an RNA in Agarose gel without formaldihyde

Running an RNA in Agarose gel without formaldihyde - RNA Techniques Forum

Running an RNA in Agarose gel without formaldihyde - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-21-2007, 05:10 PM
Pipette Filler
Points: 871, Level: 16 Points: 871, Level: 16 Points: 871, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question Running an RNA in Agarose gel without formaldihyde



Hello

Let me know how to run an RNA in Agarose Gel without formaldihyde just to check the RNA of my Plant sample.

Let me know the % of agarose and quantity of RNA and loading buffer to be added
Reply With Quote
  #2  
Old 01-13-2008, 03:52 AM
Pipette Filler
Points: 760, Level: 15 Points: 760, Level: 15 Points: 760, Level: 15
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2008
Posts: 7
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Running an RNA in Agarose gel without formaldihyde

Just run ~500ng RNA in a 0.5-0.7% TBE agarose gel as you would for DNA.
Reply With Quote
Reply

Tags
agarose , formaldihyde , gel , rna , running


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Agarose gel electrophoresis of PCR products - Method and Protocol domba PCR - Polymerase Chain Reaction Forum 12 02-24-2012 03:39 AM
Dot instead of a band on precast gel (running funny!) aditi Western Blot Forum 3 10-15-2009 09:24 PM
My DNA shoots out of the wells in my Agarose AnswerQueen DNA Techniques 3 04-16-2008 10:12 AM
uneven running pattern in agarose gel ali Protocols and Methods Forum 1 08-29-2006 11:41 AM
Nusieve agarose gel Samantha Protocols and Methods Forum 1 11-05-2004 10:32 AM


All times are GMT. The time now is 06:42 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14767 seconds with 16 queries