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RNA extraction OD 260 280 ratio Below 1.5?

RNA extraction OD 260 280 ratio Below 1.5? - RNA Techniques Forum

RNA extraction OD 260 280 ratio Below 1.5? - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 11-06-2007, 09:45 AM
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Thumbs up RNA extraction OD 260 280 ratio Below 1.5?



Hello everybody,

I'm a new member here and would like to learn a LOT, please be gentle.

Anyway, the other day I did my first ever RNA extraction but unfortunately it was no good. The OD 260/280 ratio was below 1.5, so I was wondering would I should do next. Do I scrap those and start new or is there anything I could do?

Thanks ever so much.
Edi
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Old 11-06-2007, 01:20 PM
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Default Re: RNA extraction OD 260 280 ratio Below 1.5?

Hello Edi,
sorry I moved the post to the appropriate forum Welcome to the forums we are all nice here.

I wouldn't simply scrap the RNA. Why don't you run the RNA on an agarose gel and check out the rRNA bands. Even better post here and we can help you.

Cheers!
Admin
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Old 11-06-2007, 03:57 PM
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Default Re: RNA extraction OD 260 280 ratio Below 1.5?

I never run raw rRNA on agarose, because I have time and supplies to rt-pcr amplify 16S rRNA, or b-actin RNA for quality control. I also agree with the post that says you shouldn't scrap the RNA. In all honesty the 260/280 ratio is not a good predictor of downstream results, as you can get amplification (and even sequencing) from degraded/dilute DNA/RNA samples.
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Old 11-12-2007, 02:14 AM
Edi Edi is offline
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Default Re: RNA extraction OD 260 280 ratio Below 1.5?

Thanks Admin and danfive for the tips. I got good results with the agarose gels, so I guess it's usable.
Thanks again.
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