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#1
10-01-2007, 10:25 AM
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 upper ribosomal band missing!! Hi there!! here is a puzzled person working with plant RNA: my RNA samples SOMETIMES are showing the upper ribosomal band less intense than the lower one (in TBE buffer gels), and sometimes it is even missing! however: - there are no signs of degradation - the RNA performs absolutely fine in RT-PCR experiments. - absorbance meassurements give 260/280 ratios of 1,9-2.2 It is not dependent on protocol (we use a special protocol since 11 years and it never failed untill now!), neither the samples (tried different pools), nor the buffers (tried different/new buffer solutions). We've found some peole in the net with the same problem, but nobody seems to have an answer... We need ultra-pure RNA for microarray experiments, and we need your help! Any suggestions/ideas are welcome!! thanks for it!! Nieves  |
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| band , missing , ribosomal , upper |
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