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upper ribosomal band missing!!

upper ribosomal band missing!! - RNA Techniques Forum

upper ribosomal band missing!! - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 10-01-2007, 10:25 AM
Pipette Filler
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Exclamation upper ribosomal band missing!!



Hi there!!

here is a puzzled person working with plant RNA: my RNA samples SOMETIMES are showing the upper ribosomal band less intense than the lower one (in TBE buffer gels), and sometimes it is even missing! however:
- there are no signs of degradation
- the RNA performs absolutely fine in RT-PCR experiments.
- absorbance meassurements give 260/280 ratios of 1,9-2.2

It is not dependent on protocol (we use a special protocol since 11 years and it never failed untill now!), neither the samples (tried different pools), nor the buffers (tried different/new buffer solutions).

We've found some peole in the net with the same problem, but nobody seems to have an answer...

We need ultra-pure RNA for microarray experiments, and we need your help! Any suggestions/ideas are welcome!!

thanks for it!!

Nieves
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