here is a puzzled person working with plant RNA: my RNA samples SOMETIMES are showing the upper ribosomal band less intense than the lower one (in TBE buffer gels), and sometimes it is even missing! however:
- there are no signs of degradation
- the RNA performs absolutely fine in RT-PCR experiments.
- absorbance meassurements give 260/280 ratios of 1,9-2.2
It is not dependent on protocol (we use a special protocol since 11 years and it never failed untill now!), neither the samples (tried different pools), nor the buffers (tried different/new buffer solutions).
We've found some peole in the net with the same problem, but nobody seems to have an answer...
We need ultra-pure RNA for microarray experiments, and we need your help! Any suggestions/ideas are welcome!!
thanks for it!!