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RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


gel recepie

gel recepie - RNA Techniques Forum

gel recepie - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 09-29-2007, 12:04 PM
Pipette Filler
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Default gel recepie



Hi,

Do anyone have a recipie of denature RNA gel with agarose in TBE buffer (not in MOPS). Plz give me detailed info of sample preparation too for this method.
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Old 04-08-2009, 04:27 AM
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Default Re: gel recepie

Hi Aaina

I run isolated RNA on agarose gel prepared in TBE. For TBE 1X 500 ml (DEPC water) preparation; you use:
6.057 g tris
0.292 g EDTA
3.09 g boric acid in DEPC treated water.
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