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Total RNA extraction from FFPE tissue

Total RNA extraction from FFPE tissue - RNA Techniques Forum

Total RNA extraction from FFPE tissue - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 09-28-2007, 06:54 PM
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Post Total RNA extraction from FFPE tissue



Hi All,

1. Could you suggest the best kit for the isolation of total RNA from Formalin Fixed, Paraffin-embedded (FFPE) tissue.

2. Reason for recommendation.

3. How do you check the quality total RNA obtained from FFPE tissue (as we cannot see 18S and 28S).

4. How to make sure that the RNA is degarded and not suitable for microarray analysis. For example sample collected in 2007 Vs 2000.
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Old 09-28-2007, 08:26 PM
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Default Re: Total RNA extraction from FFPE tissue

RNeasy FFPE Kit----Qiagen kit especially for FFPE.
You could also deparaffinize (heat at 60C), hydrate (graded EtOH 95%-50%), rinse in PBS, then digest with Proteinase K and either use the regular Qiagen RNeasy kit or Trizol extraction methods.

Last edited by danfive; 09-28-2007 at 08:29 PM.
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Old 10-02-2007, 02:13 PM
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Post Re: Total RNA extraction from FFPE tissue

Thanks,
How to check the qulaity of RNA for microarray analysis.
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Old 11-07-2007, 01:08 AM
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Default Re: Total RNA extraction from FFPE tissue

Tha Agilent Bioanalyzer 2100 is good if you have access to one.
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extraction , ffpe , rna , tissue , total


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