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· · · RNA Pictures 7 photos 6 comments |
· · · RNA Pictures 7 photos 6 comments | ||
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| Hi-thanks for your quick reply I am looking for both 5' UTR regions for protein interactions. Although, interesting that you ask, we are checking those regions for regulation of splicing. Right now from deletion constructs, I have narrowed one area of regulation to about 300 bp, just wanted to know how much further I need to go Tara |
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we are here to help! maybe I can get your help in the future... ![]() 300 bp is quite big although not bad... problem is there may be MANY proteins binding to this region... you could try UV-crosslinking / denaturing EMSA (for RNA-interacting proteins) and also non-denaturing EMSA in native gel (for protein complexes) Also I suggest you use smaller probes ie 50 nt (all you need are 6 constructs - may be a lot but definitely worth it to publish a good paper - 6 X 50 covers your 300bp fragment) as you will want to publish (eventually) where these proteins are binding (general region)... Last edited by moleculardude; 07-17-2006 at 07:52 PM. |
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