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Problem with RNA purification with Megaclear

Problem with RNA purification with Megaclear - RNA Techniques Forum

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Old 08-27-2007, 12:16 PM
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Question Problem with RNA purification with Megaclear



Hi guys,

I had a problem using Ambion Megaclear for the first time to purify in-vitro transcribed RNA. I ran some of the RNA on a gel before purification and it was just fine. However, when I checked the RNA on a gel after using Megaclear, it came out as a giant smear meaning it was degraded sometime along the way. For those of you who have used this kit, do you know what I did wrong?
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Old 08-27-2007, 12:17 PM
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Default Re: Problem with RNA purification with Megaclear

Hello,
That sounds like degradation. Be very careful you didn't use an old kit or a contaminated one.

I would use Roche RNA sephadex G-25 or G-50 columns they are really good although do cost a bit (25-30 columns for $120?). They are stored in 4C and are RNAse fre.

I have used them before for purification of in vitro transcribed RNA and it worked really well.
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