I run some samples from plant tissues side-by-side on a non-denaturing 2% agarose gel (in TBE) and I have some samples with higher-weight ribossomal bands than others. The intensity ratio is OK in every sample and there are no smears. What is happening? (The samples are from similar tissues from the same species!!)
Help me please. I need to know if some of this RNA is not suitable for RT-PCR!!!
see attached picture (line 1 -ladder; lane 2 (suspected sample)
What is the difference between line 3 and lines 4-8???