I'm wondering if anyone could tell me what the difference is I am seeing between my total RNA samples. The difference is seen between the Sample I extracted using a kit (Lanes 2, 4 and 5) and the samples I extract using the Trizol method (Lane 3 and 6). The kit sample shows a large band in every case (Lane 2, 4 and 5) which is not seen in the Trizol method (Lane 3 and 6). Is this DNA contamination? Also the Trizol method shows more faint bands towards the end of the gel. The kit uses glass fiber matrix columns to filter out the RNA. It says it effectively isolates RNA >200bps. Would the Trizol mehtod be more sensitive at getting lower MolecularWeight RNA than the kit (Lane 3 and 6)?
1% agarose gel of total RNA extracted from young aquatic plant leaf tissue. Lanes 2,4,5 used RNA extraction kit. Lanes 3,6 used the Trizol method. Lanes 1 and 7 are 1kb DNA ladder. Gel was visulized by staining with Ethidium Bromide.