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· · · RNA Pictures 6 photos 6 comments |
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07-13-2007, 06:31 PM
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 A short question all of you :) Hi, I have a question that I have a hard question for you that Im having trouble getting an answer to....here it goes: I have identified a gene that is not suppossed to be expressed in lower mammals than primates. I have qPCRed it in several types of tissue using several primer-sets that covers intron/exon boundaries. I have also cloned and sequenced my PCR products to confirm the experiment. So the big problem: When is a gene expressed in mammals....when you identify spliced mRNA? or do I have to do westerns?....I dont have a characterized antibody so this would delay my paper for months. Thx a lot for your time...        |
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