I have a question that I have a hard question for you that Im having trouble getting an answer to....here it goes:
I have identified a gene that is not suppossed to be expressed in lower mammals than primates. I have qPCRed it in several types of tissue using several primer-sets that covers intron/exon boundaries. I have also cloned and sequenced my PCR products to confirm the experiment.
So the big problem: When is a gene expressed in mammals....when you identify spliced mRNA? or do I have to do westerns?....I dont have a characterized antibody so this would delay my paper for months.
Thx a lot for your time...