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A short question all of you :)

A short question all of you :) - RNA Techniques Forum

A short question all of you :) - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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Old 07-13-2007, 06:31 PM
Pipette Filler
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Default A short question all of you :)



Hi,

I have a question that I have a hard question for you that Im having trouble getting an answer to....here it goes:

I have identified a gene that is not suppossed to be expressed in lower mammals than primates. I have qPCRed it in several types of tissue using several primer-sets that covers intron/exon boundaries. I have also cloned and sequenced my PCR products to confirm the experiment.
So the big problem: When is a gene expressed in mammals....when you identify spliced mRNA? or do I have to do westerns?....I dont have a characterized antibody so this would delay my paper for months.

Thx a lot for your time...
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