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|RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.|
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Running RNA Gels
I want to run some RNA on a gel... do i need those denaturing agents for RNA?
i read you need to denature the RNA first to get a proper size reading vs an RNA ladder...
please post some responses
Re: Running RNA Gels
For checking Rna integrity (unlike for Northern blot), I always run "normal" (as for dna and pcr products) 1% agarose gel, and I also use DNA loading buffer. It gives nice 28 and 18S bands (well, not always but that's not due to electrophoresis...).
Anybody else is doing that?
|gels , rna , running|
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