Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > RNA Techniques Forum
Register Search Today's Posts Mark Forums Read

RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Running RNA Gels

Running RNA Gels - RNA Techniques Forum

Running RNA Gels - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-10-2006, 04:54 AM
darkwng's Avatar
Volunteer
Points: 2,585, Level: 32 Points: 2,585, Level: 32 Points: 2,585, Level: 32
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2006
Posts: 34
Thanks: 0
Thanked 0 Times in 0 Posts
Question Running RNA Gels



I want to run some RNA on a gel... do i need those denaturing agents for RNA?

i read you need to denature the RNA first to get a proper size reading vs an RNA ladder...

please post some responses
Reply With Quote
  #2  
Old 10-06-2006, 02:14 PM
Pipette Filler
Points: 1,835, Level: 26 Points: 1,835, Level: 26 Points: 1,835, Level: 26
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2006
Posts: 16
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Running RNA Gels

For checking Rna integrity (unlike for Northern blot), I always run "normal" (as for dna and pcr products) 1% agarose gel, and I also use DNA loading buffer. It gives nice 28 and 18S bands (well, not always but that's not due to electrophoresis...).
Anybody else is doing that?
Reply With Quote
Reply

Tags
gels , rna , running


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
2D gel running problem cosmis85 Proteomics Forum 4 12-31-2009 05:26 AM
Dot instead of a band on precast gel (running funny!) aditi Western Blot Forum 3 10-15-2009 09:24 PM
Long Term Storage of SDS-PAGE Gels dnamolecule SDS-PAGE Gel Electrophoresis Forum 1 08-28-2008 06:34 AM
2D gel running problem cosmis85 Protocols and Methods Forum 0 02-21-2007 10:08 AM
Some questions about Polyacrylamide gels Fabius Protocols and Methods Forum 1 03-07-2004 09:27 PM


All times are GMT. The time now is 08:30 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11465 seconds with 16 queries