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How to Quantify RNA ?

How to Quantify RNA ? - RNA Techniques Forum

How to Quantify RNA ? - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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  #1  
Old 03-22-2007, 11:33 PM
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Default Re: RNA storage



do you know how to quantify RNA
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Old 03-26-2007, 09:49 AM
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Default Re: How to Quantify RNA ?

Hello Nkaur!

welcome to the forums!

There are several ways to quantify RNA.

1) UV spectrometry - you can assay RNA quantity by its UV absorbance at 260 nm. A Shimadzu or equivalent UV spec machine would assay RNA quantity pretty accurately

2) Run the RNA on an agarose gel and run a quantitative ladder such as Lambda digestion ladder. Make sure you stain with Ethidium Bromide to be quanitative.


Cheers
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Old 07-13-2007, 06:34 PM
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Default Re: How to Quantify RNA ?

Well...guess it depends on what he/she is working on. If we are talking about mRNA quantification of specific genes then I would recomend Northern Blot, RNase protection assays...and above all qPCR
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Old 12-30-2007, 09:10 PM
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Default Re: How to Quantify RNA ?

Just use UV absorbance!
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Old 05-21-2009, 08:04 PM
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Default Re: How to Quantify RNA ?

Quote:
Originally Posted by admin View Post
Hello Nkaur!


2) Run the RNA on an agarose gel and run a quantitative ladder such as Lambda digestion ladder. Make sure you stain with Ethidium Bromide to be quanitative.


Cheers

Hey u said its necessary to stain the gel with ethidium bromide to be quantitative..why so?
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