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| RNA Techniques Forum Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols. |
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#2
03-26-2007, 09:49 AM
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 Re: How to Quantify RNA ? Hello Nkaur! welcome to the forums!  There are several ways to quantify RNA. 1) UV spectrometry - you can assay RNA quantity by its UV absorbance at 260 nm. A Shimadzu or equivalent UV spec machine would assay RNA quantity pretty accurately 2) Run the RNA on an agarose gel and run a quantitative ladder such as Lambda digestion ladder. Make sure you stain with Ethidium Bromide to be quanitative. Cheers  |
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#3
07-13-2007, 06:34 PM
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| Re: How to Quantify RNA ? Well...guess it depends on what he/she is working on. If we are talking about mRNA quantification of specific genes then I would recomend Northern Blot, RNase protection assays...and above all qPCR |
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#5
05-21-2009, 08:04 PM
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| Re: How to Quantify RNA ? Quote:
Hey u said its necessary to stain the gel with ethidium bromide to be quantitative..why so? |
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