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nkaur 03-19-2007 10:17 PM

RNA gel
 
Hi everybody

Does somebody know which gel should be used to see RNA extracted from plant tissue.I mean I just want to see RNA in gel.

mirna2 03-20-2007 01:04 PM

Re: RNA gel
 
hi nkaur,

for simply checking plant RNA you can use a normal 1% agarose gel. good luck,

mirna

nkaur 03-21-2007 04:57 PM

Re: RNA gel
 
Hey mirna

thanks for reply.Is there no need of making formaldehude gels.I mean RNA will be safe in simple agarose gels.will it not be degraded?

mirna2 03-26-2007 07:09 AM

Re: RNA gel
 
hi nkaur,

well, if you just want to see RNA in a gel, simple agarose will usually work. it is true that the RNA may degrade, but my experience is that if you work carefully (use gloves, for example), you should be okay. using formaldehyde/MOPS gels doesn't prevent degradation of RNA, but allows better seperation of the RNA and increases the quality of your northerns.

Luisinlab 07-30-2007 02:01 PM

Re: RNA gel
 
I run some samples from plant tissues side-by-side on a non-denaturing 2% agarose gel (in TBE) and I have some samples with higher ribossomal bands than others. The intensity ration is OK in every sample and there are no smears. What is happening? (The samples are from similar tissues from the same species!!)

Help me please. I need to know if some of this RNA is not suitable for RT-PCR!!!

karlendico 07-31-2007 10:52 AM

Re: RNA gel
 
???????


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