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Biotin RNA EMSA Kit from Pierce

Biotin RNA EMSA Kit from Pierce - RNA Techniques Forum

Biotin RNA EMSA Kit from Pierce - Post and discuss RNA methods and RNA science related topics. RNA extraction, cDNA synthesis, RNA EMSA, and other RNA protocols.


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  #1  
Old 02-23-2007, 09:12 PM
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Hi !
I would like to perform bitinylated RNA (can be designed by Dharmacon, Lafayette, CO) and Pierce Lightshift kit, but I talked to technical support at pierce and they made it clear that the kit is for protein-DNA interaction and non for protein/RNA interaction use?

for those of you who use the kit for RNA EMSA, do you use a specuial buffer other that the one in the kit?

Thanks for your help,
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Old 06-04-2007, 09:01 PM
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Default Re: Biotin RNA EMSA Kit from Pierce

I am the newbie now in the same situation. Before I take the $$ plunge, can the experienced comment on how well the manual's protocol works as is for RNA EMSA? Please help!
Thanks all!
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  #3  
Old 06-05-2007, 05:32 AM
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Default Re: Biotin RNA EMSA Kit from Pierce

Yes I have gotten it to work for RNA EMSA there are a few threads with this information.

Try a search if you don't find it I can post it here.

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Old 06-05-2007, 08:07 PM
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Default Re: Biotin RNA EMSA Kit from Pierce

Thanks admin.

I guess there's no better way than to just try it myself. I'm going to try the SYBR/SYPRO EMSA kit from Molecular Probes (invitrogen) in case it happens to work; it looks far simpler but I was told it's less sensitive. We'll see.
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  #5  
Old 06-06-2007, 01:28 PM
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Default Re: Biotin RNA EMSA Kit from Pierce

Hello Accidental,

I wouldnt go with the SYBR/SYPRO... I doubt it would work but try it (make sure you can get a refund).

The Pierce EMSA kit is amazing.
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