Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > RNA Forum
Register Search Today's Posts Mark Forums Read

RNA Forum RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


RNA extraction from breast tissue

RNA extraction from breast tissue - RNA Forum

RNA extraction from breast tissue - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-05-2011, 09:11 AM
Pipette Filler
Points: 3,430, Level: 38 Points: 3,430, Level: 38 Points: 3,430, Level: 38
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2007
Posts: 9
Thanks: 0
Thanked 0 Times in 0 Posts
Default RNA extraction from breast tissue



I am looking for a propper protocol for RNA extraction from breast tissue. I did couples of trials and it didn't work. I got RNA quantity, conce= 40ng/ul 260/280= 1.98 and 260/230= 1.7. BUT when checking the quality in denaturing MOPS gel, the results were big chunck of DNA above the wells and no signs of the RNA subunit bands.

I did DNAse1 treatment, the results were the same.

I am using tissue frozen in liquid nitrogen. I tried pistle and mortor homogenization and polytron, and the results were the same.
Reply With Quote
Reply

Tags
breast , extraction , rna , tissue


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Tissue DNA Extraction Kit Orochem Article Discussion 0 07-24-2010 03:34 PM
Extraction of whole blood DNA and RNA bioteke DNA Extraction Forum 0 07-07-2010 03:33 AM
RNA extraction from human adipose tissue larissa yuri Protocols and Methods Forum 0 04-01-2009 03:03 PM
Detection, markers and prognostic/predictive significance ofdisseminated tumor cells. scimedweb@mail.com Cell Biology and Cell Culture 0 04-10-2008 04:56 AM
Business for Home.Actives Breast Program. CineBiala Forum Chemia 0 03-24-2008 12:55 AM


All times are GMT. The time now is 11:38 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11318 seconds with 16 queries