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#1
07-05-2011, 09:11 AM
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 RNA extraction from breast tissue I am looking for a propper protocol for RNA extraction from breast tissue. I did couples of trials and it didn't work. I got RNA quantity, conce= 40ng/ul 260/280= 1.98 and 260/230= 1.7. BUT when checking the quality in denaturing MOPS gel, the results were big chunck of DNA above the wells and no signs of the RNA subunit bands. I did DNAse1 treatment, the results were the same. I am using tissue frozen in liquid nitrogen. I tried pistle and mortor homogenization and polytron, and the results were the same.  |
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| breast , extraction , rna , tissue |
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