very little RNA

chichi2309 · #1 01-03-2011, 01:40 PM

Hi,
i have a problem with RNA extraction. I extract the total RNA from fresh sorted dendritic cells (between 10000 and 100000 cells) by using Qiagen rneasy mini kit, but I recover very little RNA (about 200pg/µl of RNA in total volume 30µl).
have you some tips to improve my extraction???
thank's in advance.

RNAworld · #2 01-19-2011, 03:30 AM

I think adding larger volume and warmer elution buffer (maybe 50oC) at the end will help.

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very little RNA

very little RNA - RNA Forum

very little RNA - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.