Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > RNA Forum
Register Search Today's Posts Mark Forums Read

RNA Forum RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


very little RNA

very little RNA - RNA Forum

very little RNA - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-03-2011, 02:40 PM
Pipette Filler
Points: 54, Level: 1 Points: 54, Level: 1 Points: 54, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default very little RNA



Hi,
i have a problem with RNA extraction. I extract the total RNA from fresh sorted dendritic cells (between 10000 and 100000 cells) by using Qiagen rneasy mini kit, but I recover very little RNA (about 200pg/Ál of RNA in total volume 30Ál).
have you some tips to improve my extraction???
thank's in advance.
Reply With Quote
  #2  
Old 01-19-2011, 04:30 AM
Pipette Filler
Points: 938, Level: 17 Points: 938, Level: 17 Points: 938, Level: 17
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2010
Posts: 18
Thanks: 4
Thanked 0 Times in 0 Posts
Default Re: very little RNA

I think adding larger volume and warmer elution buffer (maybe 50oC) at the end will help.
Reply With Quote
Reply

Tags
rna


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 11:37 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11118 seconds with 15 queries