very little RNA
i have a problem with RNA extraction. I extract the total RNA from fresh sorted dendritic cells (between 10000 and 100000 cells) by using Qiagen rneasy mini kit, but I recover very little RNA (about 200pg/Ál of RNA in total volume 30Ál).
have you some tips to improve my extraction???
thank's in advance.
Re: very little RNA
I think adding larger volume and warmer elution buffer (maybe 50oC) at the end will help.
|All times are GMT. The time now is 04:44 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved