very little RNA
i have a problem with RNA extraction. I extract the total RNA from fresh sorted dendritic cells (between 10000 and 100000 cells) by using Qiagen rneasy mini kit, but I recover very little RNA (about 200pg/Ál of RNA in total volume 30Ál).
have you some tips to improve my extraction???
thank's in advance.
Re: very little RNA
I think adding larger volume and warmer elution buffer (maybe 50oC) at the end will help.
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