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chichi2309 01-03-2011 02:40 PM

very little RNA
 
Hi,
i have a problem with RNA extraction. I extract the total RNA from fresh sorted dendritic cells (between 10000 and 100000 cells) by using Qiagen rneasy mini kit, but I recover very little RNA (about 200pg/Ál of RNA in total volume 30Ál).
have you some tips to improve my extraction???
thank's in advance.

RNAworld 01-19-2011 04:30 AM

Re: very little RNA
 
I think adding larger volume and warmer elution buffer (maybe 50oC) at the end will help.


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