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5x mops

5x mops - RNA Forum

5x mops - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


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Old 09-20-2010, 11:11 PM
Pipette Filler
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Default 5x mops



I am new to making 5x MOPS buffer. Has anyone made this before? If so, did it look yellow after autoclaving. Also, I had to add 19 pellets of NaOH to raise the pH to 7. Does this sound normal? I know it is a strong buffer, but this seemed like a lot.

Thanks.

by the way, this was for a 1000ml solution

Last edited by wolfman; 09-20-2010 at 11:22 PM.
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Old 10-01-2010, 11:27 PM
Pipette Filler
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Default Re: 5x mops

used the yellow MOPS with no problem
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