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I am new to making 5x MOPS buffer. Has anyone made this before? If so, did it look yellow after autoclaving. Also, I had to add 19 pellets of NaOH to raise the pH to 7. Does this sound normal? I know it is a strong buffer, but this seemed like a lot.
by the way, this was for a 1000ml solution
Last edited by wolfman; 09-20-2010 at 11:22 PM.
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