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| RNA Forum RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules. |
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#1
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| I am new to making 5x MOPS buffer. Has anyone made this before? If so, did it look yellow after autoclaving. Also, I had to add 19 pellets of NaOH to raise the pH to 7. Does this sound normal? I know it is a strong buffer, but this seemed like a lot. Thanks. ![]() by the way, this was for a 1000ml solution Last edited by wolfman; 09-20-2010 at 11:22 PM. |
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#2
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| used the yellow MOPS with no problem |
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| Thread | Thread Starter | Forum | Replies | Last Post |
| RNA gels | molecule2005 | RNA Techniques Forum | 4 | 07-30-2007 01:35 PM |
| Mops | admin | Article Discussion | 0 | 03-17-2007 04:52 AM |