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long RNA isolation

long RNA isolation - RNA Forum

long RNA isolation - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


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Old 06-08-2010, 08:55 PM
Pipette Filler
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Default long RNA isolation



Hi guys,

I have a big question in my mind, given that I am not an expert biochemist.

How difficult is the isolation of big RNAs? I'm thinking on lengths between 2 kb to 20 kb, or even larger.

Besides, these RNAs have very low abundances. Could we faithfully perform in vivo analysis of their function, like immunoprecipitations? Any help would be great.

Thanks!!

-Sakti
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