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-   -   long RNA isolation (http://www.molecularstation.com/forum/rna-forum/73122-long-rna-isolation.html)

Sakti 06-08-2010 08:55 PM

long RNA isolation
 
Hi guys,

I have a big question in my mind, given that I am not an expert biochemist. :gripe:

How difficult is the isolation of big RNAs? I'm thinking on lengths between 2 kb to 20 kb, or even larger.

Besides, these RNAs have very low abundances. Could we faithfully perform in vivo analysis of their function, like immunoprecipitations? Any help would be great.

Thanks!!

-Sakti


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