long RNA isolation
 

Hi guys,

I have a big question in my mind, given that I am not an expert biochemist. :gripe:

How difficult is the isolation of big RNAs? I'm thinking on lengths between 2 kb to 20 kb, or even larger.

Besides, these RNAs have very low abundances. Could we faithfully perform in vivo analysis of their function, like immunoprecipitations? Any help would be great.

Thanks!!

-Sakti