| | Re: denaturing polyacrilamide gel
Polyacrilamide gels have three important advantages over agarose gels:
- Better resolution: You can separate and visualize DNA bands with 1 bp of difference (i.e. a 80bp and a 79 bp band).
- Can hold bigger quantities of DNA: You can put up to 10 ug of DNA to a well (of 1 cm x 1 mm) without resolution loss.
- Purity: DNA recovered from this gels is extremely pure and can be used for delicate purposes (like sequencing).
For more info check: Sambrook J, Russell DW. 2001. Molecular Cloning: A Laboratory Manual, Ed. 3, Cold Spring Harbor, NY, US: Cold Spring Harbor Laboratory.