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Help! Rna elution

Help! Rna elution - RNA Forum

Help! Rna elution - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


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Old 04-18-2010, 03:58 PM
Pipette Filler
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Default Help! Rna elution



Hi,
I have a probalby dumb question.
I selected polyA RNA using a cellulose oligo-dT column, and RNA is then eluted using water. Why? I am asked, how does water exactly interact with teh cellulose to set the polyA RNA free?
thanks a lot.
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