Originally Posted by tannadurai
i use MOPS and formaldehyde gel to separate RNA i add EtBr in sample buffer. at begining above the well etbr expression is present i.e etbr moves opposite direction. at end of the gel it disappears may be run in to tank. whats wrong with this hw can i rectify this..
Nothing is wrong with what you're doing. You should put EtBr into the sample loading buffer and it will run towards the cathode (opposite direction of RNA/DNA). You should still be able to see the rRNA bands under UV because some of the EtBr will intercalate and run into the gel.