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Illumina BeadStudio Output and QC

Illumina BeadStudio Output and QC - RNA Forum

Illumina BeadStudio Output and QC - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


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Old 01-14-2010, 11:32 AM
Pipette Filler
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Default Illumina BeadStudio Output and QC



Hi.

I am to receive a human gene expression dataset output but illumina beadstudio. The data comprises of an AVG_DETECTION column and a DETECTION_P for each signal in every person. This p-value represents the probability that the signal was derived from a non-specific probe hybridisation (i.e. noise).

Does anyone have an insight into the best way I can quality control this data WRT the P-values? For example, should I be removing probes from my analysis if a certain number do not exceed a significance threshold of, say, 0.05? or should I be removing people?

Thanks in advance.
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