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Best method to identify all RNA sequences from extraction

Best method to identify all RNA sequences from extraction - RNA Forum

Best method to identify all RNA sequences from extraction - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


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Old 01-12-2010, 09:12 PM
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Default Best method to identify all RNA sequences from extraction



I am trying to find the most elegant way to identify all of the RNA that I obtain from an extraction - from protein produced in E. coli. My idea involves using a 3' RNA tailing kit to poly adenylate all RNA and then find some way to ligate stuff to the 5' ends and then use RT-PCR. Any other ideas?
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Old 01-13-2010, 12:33 AM
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Default Re: Best method to identify all RNA sequences from extraction

Valuable information.
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