Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum > RNA Forum
Register Search Today's Posts Mark Forums Read

RNA Forum RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


HELP!!!!454-flx

HELP!!!!454-flx - RNA Forum

HELP!!!!454-flx - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-16-2009, 03:24 AM
Pipette Filler
Points: 14, Level: 1 Points: 14, Level: 1 Points: 14, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default HELP!!!!454-flx



Anybody has experience on 454-FLX transcriptome sequence?
I want to know if itís possible to test the transcriptome difference of my experimental subject, a kind of snail without any genome information either any library information. I know I can test the sequence. Since cDNA is fragmented before sequencing, how can they translate the fragmented cDNA sequence differences into cDNA differences? I mean,how they link these fragmented cDNA together? Is it possible to perfume without any other informations?
The second question is why we construct libraries. What the role these libraries play in sequencing?
Any publication on that? I am new in this field.
Thanks a lot !
Reply With Quote
Reply

Tags
help454flx


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 10:32 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11531 seconds with 15 queries