Anybody has experience on 454-FLX transcriptome sequence?
I want to know if itís possible to test the transcriptome difference of my experimental subject, a kind of snail without any genome information either any library information. I know I can test the sequence. Since cDNA is fragmented before sequencing, how can they translate the fragmented cDNA sequence differences into cDNA differences? I mean,how they link these fragmented cDNA together? Is it possible to perfume without any other informations?
The second question is why we construct libraries. What the role these libraries play in sequencing?
Any publication on that? I am new in this field.
Thanks a lot !