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RNA and Protein From same Samples

RNA and Protein From same Samples - RNA Forum

RNA and Protein From same Samples - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


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Old 07-15-2009, 04:05 PM
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Default RNA and Protein From same Samples



Hello Everyone,

We need to do a RNA and Protein extraction from the same sample for Real Time and Western analysis.

We have a RNA extraction kit from QUIAGEN that works fine. From the first flow-through we are trying to recover the protein but without sucess.

We know that there are some kits for this but they are to expensive.

Any ideas for that? More lyse? Protein precipitation?

Thanks

Editing: I think that some component on the Rna Lysis buffer may inhibit complete lysis and protein recover. Anyone with experience on that?

Last edited by Arastho; 07-15-2009 at 05:16 PM.
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Old 07-15-2009, 05:25 PM
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Default Re: RNA and Protein From same Samples

Look into Trizol. I used that in undergrad to make cDNA for QPCR, and I am pretty sure you can also make a protein prep somehow with it.
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