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does RNA conc increase after freezing

does RNA conc increase after freezing - RNA Forum

does RNA conc increase after freezing - RNA Forum. Discuss RNA including mRNA structure and function, rRNA, tRNAs, and the biochemistry of other RNA molecules.


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Old 04-03-2009, 04:58 AM
Pipette Filler
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Default does RNA conc increase after freezing



I extracted RNa from my smaple and then used nanodrop to measure their conc and OD.I got
sample 1- 254.9ng/microL and 260/280- 2.09
sample2-211.7ng/microlitre and 260/280- 2.09
and then I froze them at -80deg for 2days.
After 2 days I measured again and the conc was
sample 1-313.4ng/microlitre and 260/280 -2.08
sample 2 - 305.8ng/microlitre and 260/280 - 2.07
what was the problem?
I used nanodrop1000
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Old 04-08-2009, 09:13 AM
Pipette Filler
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Default Re: does RNA conc increase after freezing

Maybe the RNA was not completely dissolved when you measured it for the first time? If you overdry the pellet, it may be hard to dissolve.

Cheers
Dennis
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Old 05-22-2009, 10:19 AM
Pipette Filler
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Default Re: does RNA conc increase after freezing

If you obtain different values when you measure your samples in triplicates the same day, the problem comes probably from the nanodrop. If you don't, then I would believe that your problem comes from RNA dissolution (as Pijl proposed).
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