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| Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here. |
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#1
05-19-2011, 08:15 PM
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 Gst hi guys sorry if this is being repeated.. but i wud like to know if there is any sp reason to use GST in the production of a fusion protein..are there any other systems which are also used in the production of fusion proteins.. is there a specific end like c terminal or n terminal to which the GST is fused with the vector.. thnxz  |
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#2
08-06-2011, 06:09 AM
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| Re: Gst GST improves the solubility of the expressed target protein. I use pET49b vectors with N-terminal GST and also His-tag fusion to protein of interest for bacterial protein expression. The tags are cleavable using HRV 3C protease. |
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