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Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


Gst

Gst - Recombinant Protein Forum

Gst - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


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  #1  
Old 05-19-2011, 08:15 PM
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Default Gst



hi guys

sorry if this is being repeated..
but i wud like to know if there is any sp reason to use GST in the production of a fusion protein..are there any other systems which are also used in the production of fusion proteins..
is there a specific end like c terminal or n terminal to which the GST is fused with the vector..
thnxz
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Old 08-06-2011, 06:09 AM
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Default Re: Gst

GST improves the solubility of the expressed target protein. I use pET49b vectors with N-terminal GST and also His-tag fusion to protein of interest for bacterial protein expression. The tags are cleavable using HRV 3C protease.
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