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| Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here. |
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#1
03-13-2011, 08:24 PM
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 T7-tagged protein elution dear all, I produce a trimeric complex with one T7-tagged protein. Classicaly, elution is performed using a low pH buffer. But in my case, low pH induces complex dissociation. Do you think it's possible to perform elution using a T7 petptide as for the FLAG tag ? thank you! Best regards Nico  |
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#2
08-27-2011, 05:46 PM
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| Re: T7-tagged protein elution what about engineering a protein cleavage site between the T7 tag and your protein; cleaving off your protein complex first, then cleaning up the column by low pH wash that elutes your T7 tag? |
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| elution , protein , t7tagged |
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