T7-tagged protein elution
I produce a trimeric complex with one T7-tagged protein. Classicaly, elution is performed using a low pH buffer. But in my case, low pH induces complex dissociation. Do you think it's possible to perform elution using a T7 petptide as for the FLAG tag ?
Re: T7-tagged protein elution
what about engineering a protein cleavage site between the T7 tag and your protein; cleaving off your protein complex first, then cleaning up the column by low pH wash that elutes your T7 tag?
|All times are GMT. The time now is 08:18 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved