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tavernier7 03-13-2011 08:24 PM

T7-tagged protein elution
 
dear all,
I produce a trimeric complex with one T7-tagged protein. Classicaly, elution is performed using a low pH buffer. But in my case, low pH induces complex dissociation. Do you think it's possible to perform elution using a T7 petptide as for the FLAG tag ?
thank you!
Best regards
Nico

Felicia 08-27-2011 05:46 PM

Re: T7-tagged protein elution
 
what about engineering a protein cleavage site between the T7 tag and your protein; cleaving off your protein complex first, then cleaning up the column by low pH wash that elutes your T7 tag?


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