T7-tagged protein elution
 

dear all,
I produce a trimeric complex with one T7-tagged protein. Classicaly, elution is performed using a low pH buffer. But in my case, low pH induces complex dissociation. Do you think it's possible to perform elution using a T7 petptide as for the FLAG tag ?
thank you!
Best regards
Nico

Re: T7-tagged protein elution
 

what about engineering a protein cleavage site between the T7 tag and your protein; cleaving off your protein complex first, then cleaning up the column by low pH wash that elutes your T7 tag?