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| Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here. |
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#1
03-09-2011, 08:51 AM
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 Fusion protein expression Hi, i fused 3.3 kb fusion gene in pET 28 (C),transformed in BL21 (DE3) pLysS, induced with IPTG 1 mM,4 hrs, did western blot (first gene antibody) ,only my first gene (62 KDa) expressed not the second gene (60 KDa). I should get the blot at 120 KDa size. Both of my protein is un unsoluble form.What may be the reason? Please suggest ....  |
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#2
10-18-2012, 09:45 PM
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| Re: Fusion protein expression Only about 30% of recombinant proteins expressed in e.coli are soluble from the get go. Alternatively you could purify the insoluble portion and refold the protein into it's native state. |
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| expression , fusion , protein |
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