Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Recombinant Protein Forum
Register Search Today's Posts Mark Forums Read

Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


constitutively or tunably expressed vectors

constitutively or tunably expressed vectors - Recombinant Protein Forum

constitutively or tunably expressed vectors - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-25-2011, 05:10 PM
Pipette Filler
Points: 55, Level: 1 Points: 55, Level: 1 Points: 55, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default constitutively or tunably expressed vectors



Hi, I have been trying to find a vector which will express a protein constitutively at tunable or low levels, I am strugling to find a suitable system.

Anything IPTG induced is no good because we are trying to co express an enzyme and a substrate and the substrate will be IPTG induced. We dont want high levels of the enzyme just catalytic quantities.

it doesnt necisarily have to be constitutive if we can control it at low levels, the closest I have come to finding a suitable system has been the arabinose or the proprionate induced systems but these are both problematic for NMR studies as proprionate is metabolized and arabinose is suppressed by glucose and is not versitile when it comes to cell lines.

any help would be appreciated

thanks

Tom
Reply With Quote
  #2  
Old 07-28-2012, 05:03 AM
Pipette Filler
Points: 72, Level: 1 Points: 72, Level: 1 Points: 72, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2012
Posts: 5
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: constitutively or tunably expressed vectors

nice and fresh information. I ever seen here before.c an you tell me how to make this tips and guide? thanks for your posting right here.
Reply With Quote
  #3  
Old 10-18-2012, 09:18 PM
Kneeanderthal's Avatar
Pipette Filler
Points: 68, Level: 1 Points: 68, Level: 1 Points: 68, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2012
Posts: 26
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: constitutively or tunably expressed vectors

You could try using a heat induction using the λpL/pR expression system if the temperature change isn't a problem and you can still use IPTG to induce your substrate:

PMCID: PMC2848208

Found this paper on the subject (Go to NCBI, Select PMC an type ID)

Hope this helps ya
Reply With Quote
Reply

Tags
co-expression , constitutive , constitutively , expressed , tunably , vectors


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
problems with pEarleyGate Vectors? Keith Earley Arabidopsis and Plant Biology 1 01-02-2012 02:06 PM
Highly Expressed Genes Database (HEG-DB) D.BIO - Pere Puigbo Microbiology Forum 0 10-17-2007 08:38 PM
Curved components and straight vectors Don1 Physics Forum 15 02-28-2005 05:13 AM
Curved vectors Don1 Physics Forum 13 02-26-2005 12:03 PM
Constitutively expressed genes in E. coli Anthony C. Hilton Protocols and Methods Forum 1 10-09-2003 11:40 AM


All times are GMT. The time now is 01:38 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12257 seconds with 16 queries