Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Recombinant Protein Forum
Register Search Today's Posts Mark Forums Read

Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


Any way to freeze dry without specialized equipment?

Any way to freeze dry without specialized equipment? - Recombinant Protein Forum

Any way to freeze dry without specialized equipment? - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-13-2010, 12:37 PM
Pipette Filler
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Any way to freeze dry without specialized equipment?



I am purifying a small (14 kda) His-tagged protein that is very unstable, hydrophobic, positively charged, and very sensitive to degradation, oxidation, etc. It needs to be lyophilized (freeze-dried) for storage of longer than 1 week (which preserves its activity, based on other reports). Apparently quick freezing (dry ice / ethanol) with 10-50% glycerol, then storage at -80 C results in loss of activity.

However, I have no access to a freeze-drier. We do have a vaccuum pump and can make a cold trap (dry ice / ethanol), and have a speed vac. Is there any way to use some combination of this equipment to freeze dry? Would freezing my samples to, i.e., -80C and putting them directly in a speed vac (either room temperature, or in a cold room) likely work? Any other ideas? My sample volumes are 100ul to 500ul.



Thanks for any advice!
Reply With Quote
Reply

Tags
dry , equipment , freeze , specialized


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Confused about the different principles to freeze & thaw cellsand purified protein Matthew Connelly Protocols and Methods Forum 0 07-24-2009 08:16 AM
Lab Equipment Auction eouellette Molecular Biology Products and Vendors 0 05-02-2009 09:30 PM
Pulse-Field Gel Electrophoresis Equipment TJames Protocols and Methods Forum 0 04-02-2004 03:19 PM
Pulse-Field Gel Electrophoresis Equipment TJames Yeast Forum 0 04-02-2004 03:19 PM
Used Lab Equipment at LabX labx.com Chemistry Forum 1 11-22-2003 01:37 PM


All times are GMT. The time now is 07:27 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11142 seconds with 16 queries