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Can I make glycerol stocks of transformed Rosetta/BL21 cells?
Is it possible (or advisable) to make glycerols stocks of Rosetta/BL21(DE3) cells and store them? Also, when I have single colonies of transformed cells on agar plates, how long can I store them at 4C.
Your help/suggestion is appreciated,
University of Alberta
Re: Can I make glycerol stocks of transformed Rosetta/BL21 cells?
It should be fine. I've kept BL21 and CL41 cells in glycerol stocks for storage in liquid N2 for over a year with no problems. I use a ratio of 1:1 glycerol to inoculum. As far as plates in the fridge, I've kept ioslated colonies viable for over 6 months, which is funny because my advisor told me 2-3 weeks max. The thicker the nutrient agar the better and make sure you wrap the plate with Parafilm to keep the moisture in.
|cells , glycerol , make , rosetta or bl21 , stocks , transformed|
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