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Can I make glycerol stocks of transformed Rosetta/BL21 cells?

Can I make glycerol stocks of transformed Rosetta/BL21 cells? - Recombinant Protein Forum

Can I make glycerol stocks of transformed Rosetta/BL21 cells? - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


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Old 12-11-2009, 09:18 PM
Pipette Filler
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Default Can I make glycerol stocks of transformed Rosetta/BL21 cells?



Hi All,

Is it possible (or advisable) to make glycerols stocks of Rosetta/BL21(DE3) cells and store them? Also, when I have single colonies of transformed cells on agar plates, how long can I store them at 4C.

Your help/suggestion is appreciated,

Muhammad
University of Alberta
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Old 04-04-2010, 12:28 AM
Pipette Filler
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Default Re: Can I make glycerol stocks of transformed Rosetta/BL21 cells?

It should be fine. I've kept BL21 and CL41 cells in glycerol stocks for storage in liquid N2 for over a year with no problems. I use a ratio of 1:1 glycerol to inoculum. As far as plates in the fridge, I've kept ioslated colonies viable for over 6 months, which is funny because my advisor told me 2-3 weeks max. The thicker the nutrient agar the better and make sure you wrap the plate with Parafilm to keep the moisture in.
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