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Problems with IPTG induction of PT7/lac Operon

Problems with IPTG induction of PT7/lac Operon - Recombinant Protein Forum

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Old 07-29-2009, 07:10 PM
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Question Problems with IPTG induction of PT7/lac Operon



We have problems with IPTG induction of GST from the PT7/lac Operon (Origene vector). We exactly followed the protocol provided by Origene, used BL21 (DE3) pLysS bacteria, did the induction side by side with the conventioal Tac-driven GST system, which worked great as usual, but did not get any GST expression with the Origene vector on a Coomassie gel. Has anybody encountered a similar problem? Or has any explanation? Thanks

Last edited by Pipetti13; 07-30-2009 at 06:18 PM.
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induction , iptg , operon , problems , pt7 or lac


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