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Recombinant Production using CHO (HELP)

Recombinant Production using CHO (HELP) - Recombinant Protein Forum

Recombinant Production using CHO (HELP) - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


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Old 04-24-2008, 11:22 AM
Pipette Filler
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Default Recombinant Production using CHO (HELP)



Hi there,

I am investigating the possibilty of improving recombinant proteins (rP) using a Hexokinase two construct. The idea is to up-regulate the amount of viable CHO-K1 cells i.e. the amount of rP. (exploring if this can be done transiently)

I am hoping to acquire some CHO cells (type unknown) that are producing HK2stably and this leads to my question.

1) If the Hk2 is stably being produced by CHO-K1 will I be able to directly compare productivites even though they are from different sources?


2) If I have a different CHO-Mod type i.e. different to CHO-K1 what analysis can I do using these cells???

Any I deas and help would be greatly appreciated,
Many thanks,
PlayerJS
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