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help with expressing a membrane protein

help with expressing a membrane protein - Recombinant Protein Forum

help with expressing a membrane protein - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


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Old 04-18-2008, 01:54 PM
Pipette Filler
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Default help with expressing a membrane protein



Hello all,

I am attempting to express a Type I transmembrane protein that gets most of its extracellular domain cleaved by MMPs on the cell surface, leaving just a small portion outside the membrane. My issue is that this cleaved species does not have a methionine as the N-terminal residue, since it is cleaved at a different sequence of AAs. If I wanted to express this particular form on a plasmid, how can I ensure that my recombinant protein will have the same AA sequence without an N-ter methionine?

I am thinking that cloning a canonical signal peptide onto the 5' end of my gene just before my desired sequence starts might work, anyone done something similar or have other ideas?
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