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Dynal Magnetic Beads or Sepharose for Protein Purification?

Dynal Magnetic Beads or Sepharose for Protein Purification? - Recombinant Protein Forum

Dynal Magnetic Beads or Sepharose for Protein Purification? - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


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Old 04-16-2008, 09:01 AM
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Question Dynal Magnetic Beads or Sepharose for Protein Purification?



Hiya everyone

I' new to purifying proteins and would love someone with experience


I have my protein now biotinylated (122 kDa in size).

I now want to purify the protein complex that binds to my super protein.

I liked magnetic beads however I tried Dynal M-280 Streptavidin beads and I am getting nothing compared to negative control....
I was conducting overnight binding at 4 degrees C in 50mM Tris 8.0, 150mM NaCl, 0.1% NP-40, 20% glycerol.

I was then thinking to now try sepharose beads.

Is this a good idea?

Any ideas why I may not be pulling down anything?

Your advise is greatly appreciated.
Thanks
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Old 11-18-2010, 08:14 AM
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Default Re: Dynal Magnetic Beads or Sepharose for Protein Purification?

In my experience I have found the NanoLink streptavidin beads to perform much better. I would give those a shot.
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beads , dynal , magnetic , protein , purification , sepharose , streptavidin


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