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Dynal Magnetic Beads or Sepharose for Protein Purification?
I' new to purifying proteins and would love someone with experience
I have my protein now biotinylated (122 kDa in size).
I now want to purify the protein complex that binds to my super protein.
I liked magnetic beads however I tried Dynal M-280 Streptavidin beads and I am getting nothing compared to negative control....
I was conducting overnight binding at 4 degrees C in 50mM Tris 8.0, 150mM NaCl, 0.1% NP-40, 20% glycerol.
I was then thinking to now try sepharose beads.
Is this a good idea?
Any ideas why I may not be pulling down anything?
Your advise is greatly appreciated.
Re: Dynal Magnetic Beads or Sepharose for Protein Purification?
In my experience I have found the NanoLink streptavidin beads to perform much better. I would give those a shot.
|The Following User Says Thank You to carissaseidl For This Useful Post:|
|beads , dynal , magnetic , protein , purification , sepharose , streptavidin|
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