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| Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here. |
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#1
04-16-2008, 09:01 AM
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 Dynal Magnetic Beads or Sepharose for Protein Purification? Hiya everyone I' new to purifying proteins and would love someone with experience  I have my protein now biotinylated (122 kDa in size). I now want to purify the protein complex that binds to my super protein. I liked magnetic beads however I tried Dynal M-280 Streptavidin beads and I am getting nothing compared to negative control....  I was conducting overnight binding at 4 degrees C in 50mM Tris 8.0, 150mM NaCl, 0.1% NP-40, 20% glycerol.  I was then thinking to now try sepharose beads. Is this a good idea? Any ideas why I may not be pulling down anything? Your advise is greatly appreciated. Thanks  |
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#2
11-18-2010, 07:14 AM
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 Re: Dynal Magnetic Beads or Sepharose for Protein Purification? In my experience I have found the NanoLink streptavidin beads to perform much better. I would give those a shot. |
| The Following User Says Thank You to carissaseidl For This Useful Post: | ||
admin (02-03-2012)
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| beads , dynal , magnetic , protein , purification , sepharose , streptavidin |
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