| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| Recombinant Protein Forum Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here. |
 |
 |
| | LinkBack | Thread Tools | Display Modes |
04-16-2008, 09:01 AM
| |||||
| |||||
 Dynal Magnetic Beads or Sepharose for Protein Purification? Hiya everyone I' new to purifying proteins and would love someone with experience  I have my protein now biotinylated (122 kDa in size). I now want to purify the protein complex that binds to my super protein. I liked magnetic beads however I tried Dynal M-280 Streptavidin beads and I am getting nothing compared to negative control....  I was conducting overnight binding at 4 degrees C in 50mM Tris 8.0, 150mM NaCl, 0.1% NP-40, 20% glycerol.  I was then thinking to now try sepharose beads. Is this a good idea? Any ideas why I may not be pulling down anything? Your advise is greatly appreciated. Thanks        |
| Today
| ||||
| ||||
 Sponsored Links |
|
| Tags |
| beads, protein, purification, streptavidin |
| Thread Tools | |
| Display Modes | |
|
|
Linear Mode
