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Remove Albumin Contamination from Purification Protein

Remove Albumin Contamination from Purification Protein - Recombinant Protein Forum

Remove Albumin Contamination from Purification Protein - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


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Old 02-26-2008, 12:48 PM
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Default Remove Albumin Contamination from Purification Protein



Hi im going to purify my His-tagged protein from a cell culture medium. However i just ran a SDS-PAGE and found that albumin was there as a thick band in the gel!


How will albumin affect my purification process?

I was using column from GE healthcare (His-trap FF) will albumin bind to the column? Are there any ways of removing albumin?

thanks again!

Last edited by admin; 02-26-2008 at 12:50 PM.
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Old 11-17-2008, 10:59 PM
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Default Re: Remove Albumin Contamination from Purification Protein

I too need to remove albumin from a small volume of my mAb. I know one can use Cibacon Blue dye matrix but please let me know if you find an easy way, like a one step centrifugation.
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