Refolding Insoluble Purified Proteins

[postdock]

Hello everyone,
Im trying to refold an insoluble viral glycoprotein which was expressed in E.coli, the problem is it is present in inclusion bodies.

Does anyone have experience in purifying proteins from inclusion bodies?

I want to use the protein to generate polyclonal antobodies in rabbits.

Does anyone have a protocol to refold my protein effectively and how can i check that its properly folded after refolding?

Many thanx

[danfive]

See:
"Protein expression and refolding – A practical guide to getting the most out of inclusion bodies"

---> http://www.sciencedirect.com/science...0c553175e91f24

[danfive]

And this:
"Recombinant protein folding and misfolding in Escherichia coli"
---> http://www.nature.com/nbt/journal/v2...l/nbt1029.html