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Refolding issue a cysteine rich peptide

Refolding issue a cysteine rich peptide - Recombinant Protein Forum

Refolding issue a cysteine rich peptide - Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.


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Old 01-13-2008, 09:55 PM
Pipette Filler
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Unhappy Refolding issue a cysteine rich peptide



I need to refold a cyteine rich 100 odd residue peptide expressed in E.coli. I can get high purity from inclusion bodies and passing it through a Ni-NTA column in 6M guanidium chloride but it aggregates in dialysis. I tried a few different dial cond. but I lost the majority of recombinant protein. I would need a systematic optimization starategy to get the most native/bioactive peptide at the end. Thanks for your time.
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Old 12-22-2008, 04:55 PM
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Default Re: Refolding issue a cysteine rich peptide

Hello
Try an on-column refolding on imac.

I suggest the following additives
Gluthatione Reduced / Ox
L-Arginine
Glycerol
Mannitol
Triton X100
CuSo4 ( not in IMAC, but in dilution)


check the refold databse


regards.

ed
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Old 03-04-2009, 09:04 PM
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Default Re: Refolding issue a cysteine rich peptide

i can suggest a good paper about refolding of recombinant proteins

"In vitro folding of inclusion body proteins" Rainer Rudolph and Hauke Lilie (Review)
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cysteine , issue , peptide , refolding , rich


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