Protein Expression Retain Enzyme Activity

[postdock]

Hi everyone. I am doing some new studies on protein characterisation.

I will need to prepare a lot of protein and protein that is good enough to retain enzyme activity.

I know that e.coli does not modify proteins well or fold them, especially complex enzymes.

What is the best method for large expression?

Baculovirus seems low output. Does yeast expression cut it????

[aftabac]

Well
it depends on your type of enzymes, if your enzyme work in a form without any modification then best way is to express in E.coli, and lot of procedure are avilable, and you can easily express and purify your enzymes.
but if your enzyme requires modification then you can use yeast system. now many yeast system are in use, like S.cereviseae, P.Pastoris,K.lactis etc and you can choose any of these and can express and purify your protein and can do the required assay, but if it is enzyme to be used in human, then you can use bacalovirus expression system, in this case the protein production will be not that much, but this has an advantage that the protein will be in proper folding, but this will be more expansive as compared to others. so
carefully study your enzymes, also review literature and according to requirment choose the best system.
best of luck for your work
regards
aftab