Concentrating Recombinant Proteins

[gandalfthegrey]

Hi,

I have cloned and expressed my protein. I am in the process now of purifying and concentrating the proteins now.

The problem is I lose a bit of protein due to the small size of the protein 10kda during dialysis.

Does anyone have a method of concentrating recombinant proteins without dialysis?

cheers!

[admin]

Hello there,
Happy New Year.

I would try TCA precipitation or Lyophilization however, make sure you do this on a small amount of your protein first and check to see if your protein is functional etc and you get a good amount of protein back.

Also, there are many column kits for concentration/purifying proteins including small proteins such as yours.

You can even get rid of most of the larger contaminant proteins with a column that has a low cutoff, ie only keeps proteins of size 15kda or less.
Good luck and
Cheers!

[irfan_sadiq]

hello all,
if we have detected novel protein during 2-D gel electrophoresis,how we can see its function by using biotechnology or any other possible way.